Measurement of apoptosis in heterogenous cell populations

Detection of apoptosis in a heterogenous cell population using flow cytometry.

Apoptosis induced in human leukemic cells (promyelocytic human leukemic cells HL-60, multidrug-resistant subline HL-60/VCR) and human ovarian carcinoma cells (A2780 and multidrug-resistant subline A2780/ADR) in vitro was detected by flow cytometric analysis or DNA electrophoresis. The cytofluorometric techniques utilized, i. e. detection of phosphatidylserine exposed at the outer surface of the...

Enabling Privacy-Preserving GWAS in Heterogenous Human Populations

Evolutionary Game Dynamics in Populations with Heterogenous Structures

Evolutionary graph theory is a well established framework for modelling the evolution of social behaviours in structured populations. An emerging consensus in this field is that graphs that exhibit heterogeneity in the number of connections between individuals are more conducive to the spread of cooperative behaviours. In this article we show that such a conclusion largely depends on the indivi...

Induction of apoptosis in human tumor cell lines by platelets

Introduction: It has been reported that platelets can eradicate tumor cells in vitro, although the mechanism of this effect has not been determined. The effect of platelets on the induction of apoptosis in tumor cells is largely unknown. Materials and methods: To investigate this effect, two human hematologic cell lines, K562 and Daudi, were independently faced with unstimulated and thromb...

Measurement of cell velocity distributions in populations of motile algae.

The self-propulsion of unicellular algae in still ambient fluid is studied using a previously reported laser-based tracking method, supplemented by new tracking software. A few hundred swimming cells are observed simultaneously and the average parameters of the cells' motility are calculated. The time-dependent, two-dimensional distribution of swimming velocities is measured and the three-dimen...